中国水稻科学

中国水稻科学 ›› 2012, Vol. 26 ›› Issue (5): 549-554.DOI: 10.3969/j.issn.10017216.2012.05.006

• 研究报告 • 上一篇    下一篇

利用CEL Ⅰ酶鉴定水稻广亲和基因S5位点的籼粳属性

倪深1 ,肖文斐2 ,陈红旗1,王跃星1 ,朱旭东1，*   

  1. 1中国水稻研究所 水稻生物学国家重点实验室， 浙江 杭州 310006； 2杭州市农业科学研究院 生物技术研究所， 浙江 杭州 310024；
  • 收稿日期:2011-10-20 修回日期:2011-11-16 出版日期:2012-09-10 发布日期:2012-09-10
  • 通讯作者: 朱旭东1，*
  • 基金资助:

    转基因生物新品种培育重大专项（2011ZX08001004）。

Molecular  Classification  of   indicajaponica  Rice According to     Widecompatibility Gene S5  Sequence with Endonuclease CEL Ⅰ 

NI Shen1 , XIAO Wenfei2, CHEN Hongqi1, WANG Yuexing1, ZHU Xudong 1，*   

  1. 1 State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China;  2 Institute of Biotechnology, Hangzhou Academy of Agricultural Sciences, Hangzhou 310024， China；
  • Received:2011-10-20 Revised:2011-11-16 Online:2012-09-10 Published:2012-09-10
  • Contact: ZHU Xudong1，*

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被引次数

1

摘要： 广亲和基因S5的克隆为水稻品种亲和特性的鉴定提供了分子学依据。利用PCR技术对水稻S5位点中籼、粳序列间存在差异的区段进行扩增，并结合芹菜核酸内切酶CEL Ⅰ对其进行酶切鉴定，从而区分S5不同的基因型。研究结果表明该方法能准确鉴定不同水稻品种中S5位点的籼粳特性，并能发现新的差异序列。

关键词: 水稻, 广亲和, S5基因, 籼粳属性

Abstract: Cloning of the major locus S5，  a widecompatibility gene has laid a molecular basis for identification of the widecompatibility varieties(WCV). According to the difference in S5 locus sequence among indica, japonica and WCV,  PCR was conducted and celery endonuclease CEL Ⅰ,which cleaves DNA with high specificity at sites of basesubstitution mismatch, was used to digest PCR products so as to distinguish various S5 DNA fragments. The results showed that the method is accurate in indicajaponica classification. At the same time, a new genotype on the S5 locus was found.

Key words: rice, widecompatibility, S5 locus, indica/japonica classification

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